The SF.3 is a dedicated stopped-flow accessory for the Chirascan.

The SF.3 accessory brings to Chirascan our world-leading expertise in stopped-flow design to enable circular dichroism (CD), absorbance and fluorescence stopped-flow measurements of unsurpassed sensitivity. The unit is based on our best-selling SX20 and PiStar stopped-flows, and designed to enable rapid and straightforward coupling to Chirascan: a roller-plate mount enables it to be easily located and locked into place at the exit slit of the Chirascan monochromator (in place of the sample chamber unit).

A demountable stopped-flow cell provides optical pathlengths of 2mm and 10mm and has a dead-time of 1.5ms. Cells can be changed in a few minutes; a smaller, lower dead-time, cell is available as an option. Circular dichroism (CD) and absorbance kinetics are collected using the standard Chirascan detector. Fluorescence detection is also possible with option TF.3.

SF.3 Stopped-flow accessory

Single shot data examples showing circular dichroism, absorbance and fluorescence kinetic traces.

Chirascan Circular Dichroism Spectromter with SF.3 accessory

The SF.3 stopped-flow accessory is easily mounted to the Chirascan.

Accessory Features

• Optimised for circular dichroism, absorbance and fluorescence detection without the need for reconfiguration.
  
• Straightforward benchtop configuration. Can be fitted in 10 minutes.
  
• Low sample volume requirement.
  
• Flow circuit materials suitable for anaerobic experiments and aggressive reagents.
  
• Short optical pathlength for fluorescence detection (minimises inner-filtering effect).
  
• Large ratio-mixing capability up to 25:1
  
• Comprehensive range of accessories available including sequential-mixing (SQ.3), fluorescence polarisation (FP.3) and dual fluorescence detection (DF.3).