Applied Photophysics Ltd

Chirascan

Accessory Note 6

SM.3 Scanning Emission Monochromator

The SM.3 option is a software-controlled scanning emission monochromator and light guide which couples to the fluorescence port of the sample holder. The SM.3 scanning emission monochromator and TF.3 fluorescence detector together enable the collection of high quality fluorescence emission spectra.

SM.3 Scanning Emission Monochromator
SM.3 Scanning Emission Monochromator


Fluorescence emission spectra recorded using the SEM.3 and TF.3 accessories
Fluorescence emission spectra recorded using the SM.3 and TF.3 accessories.

 

Raman scattering of water.

Fluorescence sensitivity is such that the Raman scattering of water is detectable in the example the unsmoothed fluorescence data has a S/N ratio of over 70. If this data were smoothed (for example: to enable a direct comparison with data acquired on a spectrometer using electronic filtering) the S/N ratio would increase by a large factor.




Accessory Features

  • Single diffraction grating symmetrical Czerny-Turner optical layout
  • Fibre-optic light-guide coupling between cuvette and monochromator entrance
  • Full software control of scanning experiments
  • May be operated using the standard CD channel or a dedicated fluorescence channel (option MC.3) which would also enable simultaneous CD and fluorescence signal detection capability

Other Chirascan Accessories


Chirascan Circular Dichroism Spectrometer
Chirascan Circular Dichroism Spectrometer
Chirascan Circular Dichroism Spectrometer