Applied Photophysics - For Circular Dichroism and Stopped-flow Spectroscopy

Chirascan™ Circular Dichroism Spectrometer

Chirascan™ Circular Dichroism Spectrometer

Specifications

Light source   150W Xe arc, air-cooled
150W Hg-Xe arc, air-cooled (optional)
 
       
Monochromator   F/7 double prism, both dispersive, both polarising  
       
Wavelength accuracy  

±0.2 nm (170nm - 400nm)
±0.5 nm (400nm - 900nm)

 
       
Wavelength precision  

±0.05 nm (170 - 400 nm)
±0.1 nm (400 - 800 nm)

 
       
Wavelength resolution   0.1nm throughout range.  
       
Bandwidth  

0 to 2nm at 160nm (in 0.1nm increments)
0 to 4nm at 180nm (in 0.1nm increments)
0 to 7.5nm at 200nm (in 0.1nm increments)
0 to 16nm (software limited) above 222nm

 
       
Wavelength range  

165nm - 1360nm.
Detector option for >900nm

 
       
Stray light  

<3ppm at 200nm
<8ppm at 180nm

 
       
Baseline stability   ±0.02 mo /hr  
       
Scanning modes  

Discrete sampling step-scan
Adaptive sampling step-scan

 
       
Scanning speeds   Dependent upon step-scan resolution approximately 500nm min-1 at 0.5nm step  
       
Kinetic modes  

Linear timebase
Split-linear timebases
Logarithmic timebase

 
       
Response time   Not applicable. Chirascan uses the superior method of digital oversampling which eliminates the risk of damage to data.  
       
CD full scale   ±6000 mo  
       
CD resolution   Better than 0.0001mo in 6000mo  
       
RMS noise
(All measured at 1nm BW and 2s integration time)
 

0.07m° @ 175nm
0.03m° @ 180nm
0.02m° @ 185nm
0.03m° @ 200nm
0.03m° @ 250nm
0.04m° @ 500nm
0.27m° @ 800nm
    —     @ 1000nm

 
       
Data channels  

Internal: 2 standard; up to 16 optional.

External (standard): 6 inputs for temperature (multiplexed); 3 analogue inputs; plenty of digital I/O.
 
       
Accessories (optional)  

PCS.3 Single Cell Holder with Peltier Temperature Control
PCM.3 Four Position Cell Auto-changer with Peltier Temperature Control
TT.3 Dual Syringe Titrator
TF.3 Total Fluorescence Detector
FP.3 Fluorescence Polarisation / Anisotropy Accessory
SM.3 Scanning Emission Monochromator
MCD.3 1T Permanent Magnet for Magnetic Circular Dichroism
FDCD Fluorescence Detected Circular Dichroism
MC.3 Multi-Channel Fluorescence
J.3 Julabo AWC-100 recirculating cooler for use with the Peltier
LD.3 Linear Dichroism Detector
ORD.3 Optical Rotary Dispersion Accessory
IR.3 Extended NIR Photomultiplier Detector
LT.3 Liquid Nitrogen Cryostat
SF.3 Stopped-flow accessory

 
       
Detection modes  

Circular dichroism (std)
Absorbance/transmission (std)
FDCD (std)
Fluorescence (std)
Voltage (std)
Detector HV(std)
Temperature (std)
Linear dichroism (optional)
ORD (optional)
Fluorescence polarization/anisotropy (optional)

 

 


Chirascan Circular Dichroism Spectrometer
Chirascan Circular Dichroism Spectrometer
Chirascan Circular Dichroism Spectrometer