Applied Photophysics Ltd

FP.1 Fluorescence Polarisation/Anisotropy Accessory

FP.1 extends the measurement possibilities of the SX range of stopped flow spectrometers, by providing a convenient way to record steady state and kinetic fluorescence polarisation and anisotropy data.

The FP.1 accessory has the following features:

Hardware Features

  • T-format dual channel detection, greatly reducing noise contributions from the light source.
  • Calcite excitation polariser, enabling excitation from 220nm upwards.
  • DPUV sheet collection polarisers, enabling detection of polarised fluorescence from 300nm upwards.
  • Filter holders built into collection pieces, allowing reduction of scattered excitation light.
  • Filter holder built into excitation assembly, allowing highly versatile wavelength selection and rejection.
  • Mountings for end window or side window PMTs.
  • Easily mounts onto your SHU cell block.
  • Robust construction ensures consistent polariser alignment.

Software Features

  • Polarisation, Anisotropy or Total Emission data formats available.
  • Data may be collected in raw form, processed form, or both.
  • Data collection possible in kinetic, scanning and kinetic scanning modes.
  • Automatic PMT targeting and G-factor correction built into software.
  • Offset correction enables total removal of residual scatter contributions.
  • New transform functions specifically provided for processing of raw data and inter-conversion of polarisation and anisotropy.

Technical Specifications

Wavelength range of excitation:        220nm upwards (limited by light source)
Wavelength range of detection: 300nm to 600nm+ for DPUV polarisers.
End window PMT detection range: 300nm to 600nm
Side window PMT detection range: 200nm to 850nm
Absolute accuracy: ± 0.005 Polarisation Units
Sensitivity: 2 µM Fluorescein (without offset correction)
50 nM Fluorescein (with offset correction)

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