SX20 Stopped-Flow Spectrometer Accessories
PDA.1 Photodiode Array Detector
The PDA.1 photodiode array option enables sets of time-resolved spectra to be acquired from a single stopped-flow drive. The accessory is a self-contained spectrograph which can be configured in a few seconds without the need to realign or recalibrate the instrument.
The figure (right) shows photodiode array data acquired on the SX20 with option PDA.1 and displayed here on the SX20’s Pro-Data control software.
Pro-Data also allows data to be edited in both the wavelength and time domains, or displayed as multiple or single kinetic traces.
The data shows time-resolved spectral changes covering the time range from a few ms to 20s that occur during the acid hydrolysis of the complex cation [Ni(en)3]2+. The data was acquired over a logarithmic time scale and the spectra shown here have been edited in the wavelength domain.
Global analysis of the multi-wavelength data sets using either the Applied Photophysics PC ProK or ProK II analysis packages yields more robust kinetic parameters and allows fitting to more complex reaction mechanisms than for single wavelength data.
Click here for further information about the Pro-K and Pro-K II Global Analysis packages.
Features of PDA.1 Photodiode Array
- 256-element array that acquires up to 1500 spectra per second (0.65ms integration period).
- Two wavelength ranges are available: PDA-uv:285-725nm and PDA-vis 330-1100nm (the wavelength range of PDA-UV can be extended to 200nm using the boosted deuterium light source, option UV.1).
- Can be configured in a few seconds and requires no calibration.
- Linear, logarithmic or split time bases may be selected as appropriate to the reaction.
- User selectable digital oversampling.
- The minimum 0.65ms integration time may be increased to improve sensitivity.
- Sequential-mixing experiments are supported.
- User-friendly ProData software controls all experimental aspects with straightforward data transfer to PC ProK (or ProKII) for data analysis.
- Any spectral region may be zoomed and re-zoomed for closer examination and data over discrete wavelength ranges can be selected for export or analysis.
- PC Pro-K and ProKII global analysis packages enable straightforward and fast access to robustly fitted kinetic parameters, concentration profiles and reaction component spectra.
Other SX20 Accessories
- SQ.1 Sequential mixing
- PDA.1 Photodiode array
- SEM.1 Scanning Emission Monochromator
- AM.1 Scanning Monochromator (far-UV)
- UV.1 Deuterium light source
- RC5.1 5µl cell cartridge
- FP.1 Fluorescence polarisation/anisotropy
- DD.1 Dual detection accessory
- DF.1 Dual fluorescence detection accessory
- AN.1 Anaerobic system
- QFA.1 Quench-flow adaptor
- Pro-K Global analysis for PC
- Pro-K II 2 nd Order Global analysis for PC
Click here to request a full PDF brochure for the SX20 Stopped-Flow Spectrometer and accessories.
SX20 - the ultimate gold standard in stopped-flow
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