SX20 Stopped-Flow Spectrometer Accessories - Scanning Emission Monochromator
SEM.1 Scanning Emission Monochromator
The SEM.1 Scanning Emission Monochromator comprises a second programmable monochromator to enable automated acquisition of equilibrium and time-resolved fluorescence spectra.
The standard fluorescence configuration, with the fluorescence detector mounted directly onto the cell-block, has exceptional sensitivity. This configuration employs a cut-off filter to block scattered light of the excitation wavelength such that only the fluorescence emission signal is detected. These experimental capabilities are extended with option SEM.1 which comprises a second programmable monochromator and a light guide to connect the cell-block to the second monochromator and detector as pictured (right). In this configuration the detected emission wavelength can be selected by setting the second monochromator directly from the SX20’s control software. This also enables:
- Automated acquisition of time-resolved emission spectra
- Acquisition of steady-state emission spectra
The multi-wavelength dataset below (showing refolding of lysozyme) was acquired from an automated series of stopped-flow drives (between 310nm and 400nm) using option SEM.1.
Kinetic traces for the refolding of lysozyme reacrion at wavelengths from 310nm to 400nm.
3D-plot of the same data viewed in Pro-K.
As with multi-wavelength data acquired in absorbance mode, the entire data set can be fitted simultaneously using the PC Pro-K global analysis software. Examples of fitted data are shown below.
Examples of fitted kinetic traces from the dataset above following global analysis of the whole dataset to a 2-step reaction model (A>B>C). The fitted curves are overlaid in red.
Other SX20 Accessories
- SQ.1 Sequential mixing
- PDA.1 Photodiode array
- SEM.1 Scanning Emission Monochromator
- AM.1 Scanning Monochromator (far-UV)
- UV.1 Deuterium light source
- RC5.1 5µl cell cartridge
- FP.1 Fluorescence polarisation/anisotropy
- DD.1 Dual detection accessory
- DF.1 Dual fluorescence detection accessory
- AN.1 Anaerobic system
- QFA.1 Quench-flow adaptor
- Pro-K Global analysis for PC
- Pro-K II 2 nd Order Global analysis for PC
Click here to request a full PDF brochure for the SX20 Stopped-Flow Spectrometer and accessories.
SX20 - the ultimate gold standard in stopped-flow
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