Applied Photophysics Ltd
Manufacturer and supplier of circular dichroism, stopped-flow, and laser flash photolysis spectroscopy instruments.

SX20 Stopped-Flow Spectrometer - Sequential Mixing

SX.20 Stopped-Flow Spectrometer

SQ.1 Sequential-Mixing

The SQ.1 sequential- (or double-) mixing accessory is specifically designed to study the reactivity of intermediate and transient species. Asymmetric double mixing experiments are also fully supported (e.g. for protein folding/unfolding reactions). This accessory equips the sample handling unit with two drive rams (and 4 syringes). The first drive mixes two reagents (A and B) into an aging loop and, after a user defined aging period, a second drive mixes the aged solution with a third reagent (C) in the stopped-flow cell.


SX20 flow circuit for stopped-flow

SX20 flow-circuit for sequential mixing. The diagram shows the two drive-rams, the drive syringes, the pre-mixer, optical cell and stop syringe.

SX20 Sample Handling Unit (K-SHU)

No hardware reconfiguration is required when switching between short and long aging times; the required aging time is simply entered by the user and this can be anything in the range 14ms to 1000s.

Full drive information is provided with each experiment including, drive profiles, calculated age time, drive volume per syringe, and a measurement of the dead-time. Aging times are reproducible to within 1ms.

Other SX20 Accessories

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SX20 - the ultimate gold standard in stopped-flow

SX.20 Stopped-Flow Spectrometer
SX.20 Stopped-Flow Spectrometer
SX.20 Stopped-Flow Spectrometer