Applied Photophysics - For Circular Dichroism and Stopped-flow Spectroscopy

Technical Notes

Applied Photophysics is pleased to make available various applications and technical notes, showing different aspects of our product ranges performance. Upon completion of the form, the technical notes will be emailed to you.

View publication references which cite the use of an Applied Photophysics instrument.

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Chirascan Circular Dichroism (CD) Spectrometer

  Chirascan Quick Start: Circular Dichroism Spectra
    A guide to making reliable circular dichroism measurements on the Chirascan.
  Secondary Structure Analysis of Proteins
    An introduction to using the packages for secondary structure analysis of proteins.
  Circular Dichroism Kinetics
    Using the Chirascan SF.3 stopped-flow accessory for kinetics circular dichroism measurements
  Temperature Control
    A practical guide to using the Chirascan Peltier Temperature control units for controlling sample temperature and performing temperature-ramp experiments.
  Multi-channel acquisition.
    Using the FD.3 and MC.3 options to achieve optimum measurements of fluorescence and circular dichroism.
  Fluorescence Polarization / Fluorescence Anisotropy
    Using the FP.3 option to achieve fluorescence polarization and fluorescence anisotropy measurements on the Chirascan.
  Optical Rotatory Dispersion
    Using the ORD.3 option for Optical rotatory dispersion measurements on the Chirascan.
  Steady-State Complimentary Detection Methods
    A Study of Calycenthine using absorption, circular dichroism, and fluorescence detected CD techniques.
  Magnetic Circular Dichroism
    A guide to using the MCD.3 accessory for magnetic CD measurements.
     
     
 

SX20 Stopped Flow Spectrometer

  Observation Cells
    A guide to the various options for stopped-flow observation cells.
  Photometric Accuracy
    A study showing the photometric accuracy of the SX-series spectrometers.
  Light Sources
    A guide to the various light sources available from Applied Photophysics for the SX-series spectrometers.
  Low Temperature Capability
    A guide to performing low-temperature measurement on the SX-series spectrometers.
  Anaerobic Performance
    A guide to performing anaerobic measurements using the AN.1 accessory.
  Dead Time Determination
    Determination of the dead-time of an SX-series spectrometer
  Spectra-Kinetic (multi-wavelength) acquisition.
    A guide to performing multi-wavelength kinetic measurements and performing basic global analysis with Pro-K.
  Fluorescence Kinetics
    An introduction to performing kinetics fluorescence measurements.
  Fluorescence Scanning and Kinetics
    An introduction to performing fluorescence excitation spectra and kinetics.
  Photodiode Array
    Use of the photodiode array accessory PDA.1 for high-speed multi-wavelength kinetic measurements.
  Fluorescence Polarization / Anisotropy Detection
    Using the FP.1 option to achieve fluorescence polarization and fluorescence anisotropy measurements on SX-series spectrometers.
  Dual Channel Fluorescence
    Using the DD.1 and DF.1 options to achieve dual fluorescence measurements on SX-series spectrometers.
  SQ.1 Sequential Flow Option
    A guide to sequential-flow kinetics with the SQ.1 option.
  UV.1 Deuterium Light Source Option
    Using the deuterium light source for improved U.V. performance.
  The QFA.1 Quench-flow adapter
    Using the quenched-flow adapter.
     
     
 

PiStar Circular Dichroism Stopped Flow Spectrometer

  PiStar Quick Start: Circular Dichroism Spectra
    A guide to making reliable circular dichroism measurements on the PiStar-180.
  Circular Dichroism Kinetics
    Using the PiStar-180 for kinetics circular dichroism measurements
  Secondary Structure Analysis of Proteins
    An introduction to using the packages for secondary structure analysis of proteins.
  Temperature Control
    A practical guide to using the PiStar-180 Peltier Temperature control units for controlling sample temperature and performing temperature-ramp experiments.
  MC.2 Multi-Channel Data Acquisition
    A practical guide to using the PiStar-180 Peltier Temperature control units for controlling sample temperature and performing temperature-ramp experiments.
  PDA.2 Photodiode Array Detector
    Use of the photodiode array accessory PDA.2 for high-speed multi-wavelength kinetic measurements.
  Emission Scanning and Kinetics
    Use of the emission monochromator for fluorescence measurements.
  SQ.2 Sequential-Mixing Accessory
    A guide to sequential-flow kinetics with the SQ.2 option.
  TF.2 Total Fluorescence Option
    A guide to measuring total fluorescence spectra and kinetics with the PiStar-180 TF.2 Total Fluorescence option.
  UV.2 Boosted Deuterium Light Source
    Using the deuterium light source for improved U.V. performance.
  Quench-flow adaptor
    Using the quenched-flow adapter.
     
     
 

LKS.60 Laser Flash Photolysis Spectrometer

  Quantel Brilliant B
    Laser flash photolysis with the Quantel Brilliant B laser.
  LKS60.FF Laser Flash with Flow-flash
    Using the LKS.60FF for laser flash photolysis with with flow-flash capability.
     
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