PEPTALK 

<p>CHI PEPTALK 2021</p>

We are excited to be going to this year's virtual PEPTALK conference. Come and see us.

We will be presenting a poster on: 

Beyond alpha-helix and beta-sheet: Expanding the Role of Circular Dichroism

Dates: January 19 - 21, 2020

Time: 10am - 5pm PST 

Abstract of the poster: Circular Dichroism (CD) spectroscopy has long been regarded as a low-resolution technique as its application focused on estimating the alpha-helix and beta-sheet content of proteins by secondary structure analysis of far-UV CD data.  This view of CD, though widely held, is no longer accurate as technological advances enable CD spectroscopy to provide more comprehensive structural information including near-UV CD to assess minor changes of tertiary structure. The high sensitivity of state-of-the-art CD instrumentation like Chirascan makes such data amenable to statistical analysis, allowing for a more rigorous assessment of higher order structure. This approach allows evaluating if such changes are significant or not and thus provides more confidence than old-fashioned deconvolution analysis limited to far-UV data. Using case studies, we illustrate our improvements to CD technology, including enhanced resolution in the near-UV, improved analysis of thermal stability from continuous multi-wavelength experiments, and the use of Weighted Spectral Difference to demonstrate spectral similarity, with the ultimate purpose of allowing scientists to make informed decisions based on statistically verifiable CD data.  

 

 

For more information on booking a time slot with Pavel please contact: pavel.ryzhov@photophysics.com

For more information about the conference please visit: https://www.chi-peptalk.com/