Paper of the Month: February Edition
CD spectroscopy is a powerful technique not just for the identification and distinction of different nucleic acid conformations, but also of its strandedness, as illustrated by this study: a Chirascan V100 was used here to confirm the formation of triplex DNA in the final step of a biosensor strategy based on fluorescence resonance energy transfer (FRET).
The triplex DNA was formed between a triplex forming oligonucleotide and two hairpin sequences bound to a molecular beacon for a target DNA sequence from Vibrio parahaemolyticus. As the latter is a pathogen widely distributed in seafood, replacement of less accurate and more laborious conventional methods for detection with this biosensor has potential to benefit food safety and aquaculture industry.
Tan, Li, Liao, and Liu. 2020. “An Enzyme Free Fluorescence Resonance Transfer Strategy Based on Hybrid Chain Reaction and Triplex DNA for Vibrio Parahaemolyticus.” Scientific Reports 10: 20710.
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