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Paper of the Month: February Edition

Summary:

CD spectroscopy is a powerful technique not just for the identification and distinction of different nucleic acid conformations, but also of its strandedness, as illustrated by this study: a Chirascan V100 was used here to confirm the formation of triplex DNA in the final step of a biosensor strategy based on fluorescence resonance energy transfer (FRET).

The triplex DNA was formed between a triplex forming oligonucleotide and two hairpin sequences bound to a molecular beacon for a target DNA sequence from Vibrio parahaemolyticus. As the latter is a pathogen widely distributed in seafood, replacement of less accurate and more laborious conventional methods for detection with this biosensor has potential to benefit food safety and aquaculture industry.

 

Citation:

Tan, Li, Liao, and Liu. 2020. “An Enzyme Free Fluorescence Resonance Transfer Strategy Based on Hybrid Chain Reaction and Triplex DNA for Vibrio Parahaemolyticus.” Scientific Reports 10: 20710.

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Digital Object Identifier:

doi.org/10.1038/s41598-020-77913-2


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