Webinar: Bilayer-Mediated Regulation of Membrane Protein Function by Small Molecules
Small molecules, such as drugs, interact with membrane proteins directly and will also modify the lipid bilayer. This concept can be exploited to give valuable insight into the cytotoxicity of vast libraries of compounds. Here’s an opportunity to attend a two-part series of live webinars discussing the concept of bilayer modification by small molecules and how you can utilise it to characterise the potency of potential toxins. The two 30-minute sessions will include time for questions on the regulation of membrane protein function. Register now!
Webinar Title: Bilayer-Mediated Regulation of Membrane Protein Function by Small Molecules
Learn more about using single-mixing stopped-flow spectrofluorometry to quantify the bilayer-modifying potency of bioactive molecules.
Membrane proteins are coupled to their host lipid bilayer through hydrophobic interactions that minimise the exposure of hydrophobic surfaces to water. Consequently, membrane protein conformational changes that involve the bilayer-spanning domains will alter the lipid organisation adjacent to the protein. This lipid reorganisation has an associated energetic cost, which becomes the bilayer contribution to the energy change associated with the conformational transition. The bilayer contribution varies as a function of the host bilayer’s material properties (thickness, intrinsic curvature, and the associated elastic moduli); membrane proteins’ conformational preference thus will vary with changes in bilayer elastic properties. Amphiphiles, including many biologically active compounds, are potent modifiers of bilayer elasticity meaning that the energetic coupling between membrane proteins and their host bilayer provides for a mechanism by which amphiphilic drugs regulate membrane protein function without direct binding to the proteins in question.
This webinar will focus on: first, the basics of bilayer-mediated regulation of membrane protein function; and second, how to quantify the bilayer-modifying potency of amphiphiles using single-mixing stopped-flow spectrofluorometry.
Date: 18th March 2021
Time: 11:00 EDT | 15:00 GMT
Presented by: Olaf S. Andersen, M.D.
Professor of Physiology and Biophysics
Weill Cornell Medical College