Part 2: Webinar: Mechanisms underlying drug-mediated regulation of membrane proteins: Using sequential mixing stopped-flow to study the regulation of membrane protein function
Small molecules, such as drugs, interact with membrane proteins directly and will also modify the lipid bilayer. This concept can be exploited to give valuable insight into the cytotoxicity of vast libraries of compounds. Here’s an opportunity to attend a two-part series of live webinars discussing the concept of bilayer modification by small molecules and how you can utilise it to characterise the potency of potential toxins. The two 30-minute sessions will include time for questions on the regulation of membrane protein function.
Webinar Title: Mechanisms underlying drug-mediated regulation of membrane proteins: Using sequential mixing stopped-flow to study the regulation of membrane protein function
Membrane proteins are key targets in the pharmacological regulation of cell function. Underlying mechanisms governing drug downstream effects are often not clearly understood as off-target effects of many drugs involve the regulation of multiple membrane proteins. The plurality of protein targets that a drug can affect at the same concentration suggests a common, non-specific, mechanism. Membrane proteins are tightly coupled to the host lipid bilayer, therefore, changes in lipid bilayer properties (i.e. thickness, curvature, elasticity) regulate protein function. Most drugs are amphiphiles, being able to partition into and alter lipid bilayer properties. We study the role of drug-mediated changes in bilayer properties in their effects on membrane proteins.
In this webinar, we present our results showing regulation of a prototypical ion channel, KcsA, first by changes in bilayer thickness and second by a set of drugs that vary in their bilayer modifying potency as sensed by gramicidin channels.
Using sequential-mixing stopped-flow spectrofluorometry we interrogate different gating steps of KcsA in the presence of drugs in different bilayer environments, to show that drugs indeed can regulate membrane protein function via the bilayer. Finally, we present tools and strategies for parsing out whether mechanisms for the observed drug effects are mediated by the lipid bilayer or direct drug-protein interactions.
Date: 8th April 2021
Time: 11:00 EDT | 16:00 BST
Presented by: Radda Rusinova, Ph.D.
Assistant Professor of Research in Physiology and Biophysics
Department of Physiology and Biophysics
Weill Cornell Medicine