Monitor changes in fluorescence
Controlled through Chirascan software, the CCD fluorometer generates emission spectra in seconds providing CD, absorbance and fluorescence data in a single experiment.
Dedicated Chirascan accessories offer the flexibility required to support
multiple research projects.
Increase capacity and productivity
Controlled through Chirascan software, the 6-cell turret enables analysis of up to six samples or 6 thermal denaturation data sets per run. Precision Peltier temperature control and magnetic stirring provide optimal conditions for thermal denaturation (temperature ramp) experiments.
TITRATOR AND pH PROBE
Monitor concentration- and pH-dependent changes in CD, fluorescence or absorbance
Fully automated and controlled through Chirascan software, this dual syringe titration system systematically varies concentration of solution while maintaining constant volume. The optional pH probe enables measurement of sample pH in situ.
LD COUETTE CELL
Gain insight into conformation and relative orientation of molecules
The LD Couette Cell enables study of macromolecular structures and their interactions e.g. DNA-ligand binding or orientation of membrane proteins. These structures are either intrinsically oriented or can be oriented during an experiment.
Characterize fast reactions, complement CD spectra with kinetic information
Using the world-leading design of the stopped-flow accessory enables study of reactions in solution and determination of rate constants. Changes in CD, absorbance, fluorescence or fluorescence polarization can be monitored as a function of time.
INTEGRATING SPHERE AND
SOLID SAMPLE HOLDER
See changes in chirality of solid state samples
The integrating sphere contains a large reflecting area to ensure high signal:noise when collecting CD data from highly scattering samples such as solids and powders.
A wheel in the solid sample holder enables rotation of transparent samples such as KBr discs to eliminate birefringence effects.
A complementary technique to CD
When the nature of the sample limits the capabilities of CD analysis — ORD allows the characterization of chiral molecules at high concentrations, where absorption bands are obscured by solvent or buffer salt absorption, or when measuring at wavelengths where a chiral molecule does not necessarily absorb light.
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CIRCULARLY POLARIZED LUMINESCENCE
Examine the excited states of chiral molecules
With a photoelastic modulator as analyser, the CPL accessory monitors the chirality of fluorescence emission.
Compatible with the standard Single Cell Peltier Holder and fully integrated in the Chirascan software so that CPL and conventional CD measurements are possible with the same setup.
A FLUORESCENCE ANISOTROPY DETECTOR to characterize ligand binding events.
A MAGNETIC CD accessory for study of molecules such as metalloproteins.
A NEAR IR EXTENSION KIT to expand scanning range of a Chirascan V100 to 1700 nm.
Please contact your Applied Photophysics representative to discuss requirements and system compatibility.
Cuvettes suitable for the most commonly encountered analytical conditions are supplied with Chirascan V100. An extensive range of cuvettes is available to provide full flexibility for further optimization of sample concentration and absorbance if required. All cuvettes are manufactured from far-UV quartz to enable analysis of the secondary structure of biomolecules.