Achieve highest sensitivity and accuracy

Since their introduction in 2005, Chirascan™ systems have continued to feature in thousands of peer-reviewed publications covering a wide range of research areas. Chirascan V100 now offers the increased sensitivity and accuracy preferred for CD analysis of biomolecules.

  • Avalanche photodiode detector enhances sensitivity
  • Increased signal:noise compared to conventional photomultiplier
  • Accurate normalization from simultaneous measurement of absorbance and CD

Increased sensitivity when sample is limited
 

Chirascan V100 (avalanche photodiode detector)
Chirascan (photomultiplier)

Tertiary structure of lysozyme – raw data, no smoothing, 10 min. baseline / 10 min. sampling, n=3 scans, 0.5 nm step, 10 mm pathlength, spectra offset for clarity

Increased sensitivity: faster measurements for thermal studies or photolabile samples

Chirascan V100 (avalanche photodiode detector)
Chirascan (photomultiplier)

Tertiary structure of lysozyme – raw data, no ­smoothing, baseline corrected, n=3 scans, 1 nm step, 10 mm ­pathlength, spectra offset for clarity

  • Accurate CD values across entire wavelength range
  • Overcome challenges of chemical calibration
  • Optics-based, multiwavelength calibration

Conventional chemical calibration methods require considerable skill in preparation. Standards, such as ­camphor-10-sulfonic acid (CSA), are unstable, photolabile and hygroscopic. In addition, single wavelength ­calibration (290.5 nm) assumes the same linear response at all ­wavelengths.

The optics-based, multiwavelength calibration method used in Chirascan V100 and Chirascan Q100 overcomes these challenges. The correct calibration is applied to every wavelength to yield accurate CD values.