Used to complement CD and absorbance information, the Chirascan CCD fluorometer provides orthogonal information on tertiary structure of proteins.
Acquire additional structural information
- The addition of tertiary structure, assessed by fluorescence, to the secondary and tertiary structure assessed by far-and near-UV CD respectively.
- Measuring excitation and/or emission spectra
- Using intrinsic protein fluorescence emission and extrinsic fluorescent dyes
Acquire additional stability/folding information
- Perform isothermal and thermal denaturation experiments