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Co-Linear Sample Excitation

Measure dilute, weakly absorbing solutions that require laser excitation with longer pathlength cells.

This accessory enables laser excitation to be co-linear with the analyzing light source i.e. along the optical axis of the spectrometer. The adjustable mini-rail is set to subtend an angle of 78° to the spectrometer axis and is used as the guide for the incoming laser beam. A beam splitter steers the laser light along the optical axis.

Diffuse Reflectance

Measure opaque samples such as films attached to a vertical sample stage or powders in a cuvette

The modular design of the LKS80 sample housing optics facilitates the exchange of an existing lens holder and thermostated sample mount with the diffuse reflectance optical unit. The laser is incident on the sample at an oblique angle with the probe beam at normal incidence. Diffuse reflected light is then collected at an angle between the probe and laser beams. The vertical or lateral position of the sample is easily adjusted. Holders for opaque films and powder-filled cuvettes are supplied as standard.

Near IR Absorbance Detector

Detect transient absorbance changes up to 1650nm.

This InGaAs photodiode detector enables measurements using continuous or pulsed analysing light with a fast rise time (1.8 ns), high bandwidth (200 Mhz) and high sensitivity. A 1000 nm blazed, holographic grating is included.

NIR-PMT Module

Study reactions involving singlet oxygen emission - measure laser induced fluorescence in the NIR region

The detector is contained in a thermally insulated sealed-off housing evacuated to a high vacuum. An internal thermoelectric cooler eliminates the need for liquid nitrogen and cooling water - no external vacuum pump is required. The interface includes a detector support and a 1 micron diffraction grating with a working range of 700 to 1700nm.

Programmable Monchromator

Protect biological samples from photodegradation

To ensure that photosensitive samples are irradiated only by the analytical wavelength, an additional programmable monochromator can be easily located between the analyzing light source and the sample chamber.

This approach provides greater control and flexibility when compared to using bandpass filters. For example, by enabling acquisition of time-resolved transient absorbance spectra via an automated series of acquisitions over a range of analytical wavelengths.

Slow Flash

Record weakly absorbing, longer lived transient species

Suitable for measurements over millisecond timescales and longer, this accessory uses a built-in transient recorder with high bit resolution, an R928 photomultiplier and a detector card to provide a maximum sampling rate of 100,000 points per second (one point per 10 µs). Full acquisition tools, including split time base, 4 decade logarithmic time base, and digital oversampling are provided.

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Pro-KIV Kinetic Global Analysis

For analysis of multiwavelength kinetics

Pro-KIV Kinetic Global Analysis enables global analysis of data obtained when studying wavelength-dependent reaction kinetics or time-dependent spectra. Multiwavelength data is globally analyzed by fitting the entire dataset to a given reaction model using non-linear regression analysis.

Singular value decomposition (SVD) on multiwavelength data can identify where the main spectral changes are occurring. Isolating those components that contribute only random noise to the data can improve data quality.
The software may also be used to fit other kinetic data such as single wavelength absorbance and fluorescence data or provide kinetic data simulations based on a given model. Models ranging from simple one step reactions to highly complex reaction schemes are easily entered into the software.

In addition, concentration profiles and calculated spectra for each reaction component provide clear evidence and support for the presence of short lived reaction intermediates.