Tertiary structure of lysozyme – raw data, no smoothing, 10 min. baseline/10 min. sampling, n=3 scans, 0.5 nm step,
10 mm pathlength, spectra offset for clarity
- Avalanche photodiode detector enhances sensitivity
- Increased signal:noise compared to conventional photomultiplier
- Accurate normalization from simultaneous measurement of absorbance and CD
- Accurate CD values across entire wavelength range
- Overcome challenges of chemical calibration
- Optics-based, multiwavelength calibration
Conventional chemical calibration methods require considerable skill in preparation. Standards, such as camphor-10-sulfonic acid (CSA), are unstable, photolabile and hygroscopic. In addition, single wavelength calibration (290.5 nm) assumes the same linear response at all wavelengths.
The optics-based, multiwavelength calibration method used in Chirascan V100 and Chirascan Q100 overcomes these challenges. The correct calibration is applied to every wavelength to yield accurate CD values.