Stopped Flow Applications

Stopped-flow spectroscopy plays a key role in deepening our understanding of reaction mechanisms and molecular structure. From the RX2000 accessory used as a teaching aid and to extend the capability of a UV or fluorescence spectrometer through to the high-performance SX20 systems, stopped-flow solutions from Applied Photophysics are used in research labs throughout the world. Applications include investigations of protein-protein interactions, ligand binding, electron transfer, fluorescence resonance energy transfer (FRET), protein folding, as well as enzyme, chemical or coordination reactions.

Reactions are followed in solution over timescales in the range of one millisecond to hundreds of seconds. The resulting data enable researchers to determine reaction rates, reveal the complexity of a mechanism, gain information about short-lived reaction intermediates and more.