System Upgrades

For unmatched performance and productivity

Save days of operator time and generate objective statistically-validated data.

Upgrading to a Chirascan Q100 system will transform the productivity of a high performance Chirascan V100 system (and earlier systems such as Chirascan-plus)

Save days of operator time:
Assuming a seven hour working day and a set-up time of 30 minutes, the Chirascan Q100 system can analyze 48 buffer-sample pairs over 24 hours. In comparison, an experienced operator can process up to 14 samples per day using a manual system.

System upgrades are performed by a fully-trained service engineer. Integration of new equipment and software followed by a comprehensive service of existing hardware ensures that the performance of the new system reaches required standards. Installation is followed by a basic training program to familiarize users with their new system.

A team of experienced application specialists provide continuing support to assist with application-related enquiries as well as the provision of basic and advanced training upon request.

Contact your local Applied Photophysics representative for more details.

Upgrade from Chirascan to Chirascan V100

When sensitivity and accuracy are key.

Maximize the performance of a Chirascan system by upgrading to a Chirascan V100 system and transform system performance.

Key elements of this comprehensive upgrade include:

  • Replacement of the photomultiplier with an avalanche photodiode detector to enhance sensitivity, increase signal:noise and enable accurate normalization from simultaneous measurement of absorbance and CD
  • Update of monochromator optics and beam optimization for flatter, more stable CD baselines
  • Update to optics-based, multiwave length calibration to ensure accurate CD values across the entire wavelength range and overcome challenges of chemical calibration

Increased sensitivity when sample is limited
 

Chirascan V100 (avalanche photodiode detector)
Chirascan (photomultiplier)

Tertiary structure of lysozyme – raw data, no smoothing, 10 min. baseline / 10 min. sampling, n=3 scans, 0.5 nm step, 10 mm pathlength, spectra offset for clarity

Increased sensitivity: faster measurements for thermal studies or photolabile samples

Chirascan V100 (avalanche photodiode detector)
Chirascan (photomultiplier)

Tertiary structure of lysozyme – raw data, no ­smoothing, baseline corrected, n=3 scans, 1 nm step, 10 mm ­pathlength, spectra offset for clarity

Conventional chemical calibration methods require considerable skill in preparation. Standards, such as ­camphor-10-sulfonic acid (CSA), are unstable, photolabile and hygroscopic. In addition, single wavelength ­calibration (290.5 nm) assumes the same linear response at all ­wavelengths.

The optics-based, multiwavelength calibration method used in Chirascan Q100 overcomes these challenges. The correct calibration is applied to every wavelength to yield accurate CD values.

Update of Chirascan control software and addition of an active nitrogen management system (if not already present) to regulate purge gas consumption and enable scheduled start-up/shutdown of lamp and nitrogen supply
Inclusion of global thermodynamic analysis software to derive melting points and enthalpies from multiwavelength, thermal denaturation experiments

The standard upgrade package is compatible with all Chirascan instruments and accessories installed after 2005. Some older models (running Windows XP) may require an additional USB electronics upgrade for compatibility with Windows 7,8 or 10. System configurations may vary according to age.

Contact your local Applied Photophysics representative to discuss your specific requirements.

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