Chirascan™ Q100 — Circular Dichroism Spectrometer

Unmatched productivity and performance

Chirascan Q100 provides detailed insight into the Higher Order Structure (HOS) characteristics of complex biomolecules.

Reproducible robotics and high performance circular dichroism spectroscopy combine to generate quality data compatible with the most stringent statistical analysis methods. The result: objective, statistically-validated HOS comparisons.

Fully integrated for unattended operation, Chirascan Q100 saves days of operator time, analyzing 48 buffer-sample pairs over 24 hours whereas an experienced operator processes up to 14 samples per day on a manual system.

Learn more about circular dichroism and HOS of biomolecules

Ready to run

MOLECULAR SIEVE,
ACTIVATED CHARCOAL FILTER

  • Removes common gas impurities

ACTIVE NITROGEN
MANAGEMENT SYSTEM

  • Regulates purge gas consumption
  • Software-controlled

AIR-COOLED XENON LAMP

  • Software-controlled
  • Up-time recorded

MONOCHROMATOR

  • Produces horizontally, linearly polarized ­monochromatic light
  • Dual polarizing prisms maximize light ­throughput

PHOTOELASTIC MODULATOR

  • Converts horizontally polarized light to ­circularly polarized light. ­Alternates between left- and right-handed circular polarized light

INTEGRATED AUTOSAMPLER

  • Eliminate sample handling errors
  • Precise liquid handling and reproducibility
  • Temperature-controlled storage maintains sample integrity

TEMPERATURE-CONTROLLED
SAMPLE CHAMBER

  • Consistent analytical conditions
  • Continuous temperature ramps (single sample mode)

WATER CIRCULATOR

  • Dissipates heat from sample chamber and sample storage Peltiers

AVALANCHE PHOTODIODE DETECTOR

  • Highest sensitivity (high signal: noise)

OPTICS-BASED, MULTIWAVE­LENGTH CALIBRATION

  • For CD accuracy at every wavelength

FIXED FLOW CELLS

  • Eliminates errors of cuvette handling
  • Recognized by Chirascan Control to select run/wash/dry protocols
  • Choice of pathlength to optimize ­concentration and absorbance

CUVETTES AND HOLDERS

  • Selected for far- and near-UV CD analysis of biomolecules (single sample mode)
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HOS Comparison Software

Data obtained on a Chirascan Q100
  • HOS Comparison software enables calculation of weighted spectral difference scores for reference and sample datasets – converting spectra into numerical data.
  • These scores are then exported and used to generate a quality range test or equivalence test as recommended by the FDA.

Optimized for Chirascan Q100

  • Confirming the statistical significance of any detected change in HOS requires a minimum of five sample replicates to enable robust analysis*. HOS comparison software is therefore optimized for analysis of the raw (unsmoothed) data generated on a Chirascan Q100 system to ensure

  • An ability to detect minor changes in HOS
    • An autosampler and flow cell combine to increase reproducibility by eliminating variability due to manual handling of samples and cuvettes
    • An avalanche photodiode detector achieves a sensitivity far greater than a conventional photomultiplier detector
  • An ability to increase number of replicates without increasing demands on operator time

The software includes comparison methods and statistics associated with several statistical methods. However, we recommend calculating z-scores to quantify the similarity between a single spectrum and the reference dataset for each sample using ‘weighted spectral difference’ (Dinh, Nikita N., et al. Anal. Biochem. 464 (2014): 60-62).

For more information about quality range tests consult guidance documents from the relevant regulatory agencies.  For example: Statistical Approaches to Evaluate Analytical Similarity – Guidance for Industry, CDER, FDA

*Analyses with three or four replicates can be sufficient for proof of principle studies, but statistical analysis with less than three replicates is not mathematically possible

Note: t-tests are not recommended for HOS comparisons and are absent from the statistical approaches recommended by regulatory agencies at this time.

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Control Software

  • Easily defined run parameters and store routine protocols
  • Saves time with scheduled start-up/shutdown of lamp and N2 supply
  • Fail-safe lamp switch-off if N2 flow drops
  • Ensures O2-free conditions with N2 purge
  • Recognizes flow cell to select optimal run/wash/dry protocol
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