Chirascan Q100 Product Information

Unmatched productivity and performance

Chirascan Q100 provides detailed insight into the Higher Order Structure (HOS) characteristics of complex biomolecules.

Reproducible robotics and high performance circular dichroism spectroscopy combine to generate quality data compatible with the most stringent statistical analysis methods. The result: objective, statistically-validated HOS comparisons.

Fully integrated for unattended operation, Chirascan Q100 saves days of operator time, analyzing 48 buffer-sample pairs over 24 hours whereas an experienced operator processes up to 14 samples per day on a manual system.

Learn more about circular dichroism and HOS of biomolecules

Ready to run

MOLECULAR SIEVE,
ACTIVATED CHARCOAL FILTER

Removes common gas impurities

ACTIVE NITROGEN
MANAGEMENT SYSTEM

Regulates purge gas consumption
Software-controlled

AIR-COOLED XENON LAMP

Software-controlled
Up-time recorded

MONOCHROMATOR

Produces horizontally, linearly polarized monochromatic light
Dual polarizing prisms maximize light throughput

PHOTOELASTIC MODULATOR

Converts horizontally polarized light to circularly polarized light. Alternates between left- and right-handed circular polarized light

INTEGRATED AUTOSAMPLER

Eliminate sample handling errors
Precise liquid handling and reproducibility
Temperature-controlled storage maintains sample integrity

TEMPERATURE-CONTROLLED
SAMPLE CHAMBER

Consistent analytical conditions
Continuous temperature ramps (single sample mode)

WATER CIRCULATOR

Dissipates heat from sample chamber and sample storage Peltiers

AVALANCHE PHOTODIODE DETECTOR

Highest sensitivity (high signal: noise)

OPTICS-BASED, MULTIWAVELENGTH CALIBRATION

For CD accuracy at every wavelength

FIXED FLOW CELLS

Eliminates errors of cuvette handling
Recognized by Chirascan Control to select run/wash/dry protocols
Choice of pathlength to optimize concentration and absorbance

CUVETTES AND HOLDERS

Selected for far- and near-UV CD analysis of biomolecules (single sample mode)

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HOS Comparison Software

Data obtained on a Chirascan Q100

HOS Comparison software enables calculation of weighted spectral difference scores for reference and sample datasets – converting spectra into numerical data.
These scores are then exported and used to generate a quality range test or equivalence test as recommended by the FDA.

Optimized for Chirascan Q100

Confirming the statistical significance of any detected change in HOS requires a minimum of five sample replicates to enable robust analysis*. HOS comparison software is therefore optimized for analysis of the raw (unsmoothed) data generated on a Chirascan Q100 system to ensure
An ability to detect minor changes in HOS
- An autosampler and flow cell combine to increase reproducibility by eliminating variability due to manual handling of samples and cuvettes
- An avalanche photodiode detector achieves a sensitivity far greater than a conventional photomultiplier detector
An ability to increase number of replicates without increasing demands on operator time

The software includes comparison methods and statistics associated with several statistical methods. However, we recommend calculating z-scores to quantify the similarity between a single spectrum and the reference dataset for each sample using ‘weighted spectral difference’ (Dinh, Nikita N., et al. Anal. Biochem. 464 (2014): 60-62).

For more information about quality range tests consult guidance documents from the relevant regulatory agencies. For example: Statistical Approaches to Evaluate Analytical Similarity – Guidance for Industry, CDER, FDA

*Analyses with three or four replicates can be sufficient for proof of principle studies, but statistical analysis with less than three replicates is not mathematically possible

Note: t-tests are not recommended for HOS comparisons and are absent from the statistical approaches recommended by regulatory agencies at this time.

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