Beyond α-helix and β-sheet - detect changes in secondary and tertiary structure
Today’s Chirascan circular dichroism spectrometers contribute to a deeper understanding of biomolecular characteristics, mechanisms and interactions by revealing far more about changes in higher order structure (HOS).
Analyzing proteins in solution reveals changes to their structural and thermodynamic properties. Application examples include:
- Confirmation of structural integrity
- Detection of minor changes in secondary and tertiary structure
- Determining the effect of site-directed mutations on folding/unfolding (stability)
- Monitoring response to changes in temperature or pH
- Revealing the effect of denaturing agents or interaction partners.
Gain insight and detect changes in secondary and tertiary structure
- Detect minor differences under native or stressed conditions
- Characterize protein stability
- Determine response to thermal or chemical changes
- Determine structural and thermodynamic properties
- Study folding and unfolding mechanisms.
